Composite

Part:BBa_K3052032

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-14)


Light-fluoresce circuit (GFP) - RXY

This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. The variables of this two-phases can be calculated by measuring the fluoresce intensity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6036
    Illegal NheI site found at 6059
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
    Illegal NotI site found at 4866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 5391
    Illegal BglII site found at 7596
    Illegal BamHI site found at 5173
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
    Illegal NgoMIV site found at 7149
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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